Lineage-specific genetic ablation

Author: fpdk

August undefined, 2024

Nettet29. jul. 2012 · Genetic ablation of ADAM12(+) cells, or knockdown of ADAM12, is sufficient to limit generation of profibrotic cells and interstitial collagen accumulation. Nettet15. mar. 2024 · In this present study, we performed bulk RNA-sequencing (RNA-seq) in hESC-derived articular and growth plate cartilage to identify lineage-specific gene expression, and compared them to developing fetal cartilage. We also performed ATAC-sequencing (ATAC-seq) to define the accompanying regulatory landscapes in hESC …

Generation of a novel mouse strain with fibroblast-specific …

Nettet29. jul. 2012 · Dulauroy, S., Di Carlo, S., Langa, F. et al. Lineage tracing and genetic ablation of ADAM12 + perivascular cells identify a major source of profibrotic cells during acute tissue injury. Nettet2. nov. 2024 · Lineage-specific gene families may arise from evolutionary innovations such as de novo gene origination, or may simply mean that a similarity search program failed to identify more distant homologs. A new computational method for modeling the expected decay of similarity search scores with evolutionary distance allows distinction … romantic good night prayer for her

Cancers Free Full-Text Organotypic Culture of Acinar Cells for …

Nettet31. jul. 1987 · Cell lineage ablation in transgenic mice by cell-specific expression of a toxin gene. A method of deleting specific cell lineages has been developed that … Nettet5. aug. 2013 · Main Text. Recent advances in cellular reprogramming have re-awakened interest in lineage-specification mechanisms and challenged views about the very nature of commitment itself [1].From a molecular viewpoint, lineage identity results from the accumulation of stable lineage-specific transcription factor complexes at the … Nettet20. apr. 2024 · Specific genetic targeting of GPCMs is the basis for accurately evaluate the cell fate and function during heart repair and regeneration. ... A Cre-inducible diphtheria toxin receptor mediates cell lineage ablation after toxin administration. Nat Methods. 2005; 2:419–426. doi: 10.1038/nmeth762 Crossref Medline Google Scholar; 34. romantic gothic wedding cake

Gene Deletion - an overview ScienceDirect Topics

Cell Ablation - an overview ScienceDirect Topics

Nettet1. jan. 1995 · DT-A catalyzes the ADP-ribosylation of elongation factor 2, causing an 32. Cell-Specific Ablation in Plants 441 inhibition of protein synthesis and subsequent cell death. The DT-B chain directs internalization to most eukaryotic cells via a specific membrane receptor. Addi- tionally, the DT-A chain does not include a signal peptide … Nettet1. mar. 2015 · Professional biomedical scientist with 14+ years experience in Neuroscience research, including in the pharmaceutical industry, … romantic good night wishesNettetLineage tracing and genetic ablation of ADAM12(+) perivascular cells identify a major source of profibrotic cells during acute tissue injury. Profibrotic cells that develop upon … romantic good night sweet dreams quotes

"Nettet23. mar. 2024 · a, Schematic showing specific endocardium-derived fibroblast ablation by dual recombinase-mediated genetic approach using Nfatc1-Dre;Col1a2-CreER;R26-LSL-RSR-tdT-DTR mice. b , Schematic showing ... " - Lineage-specific genetic ablation

Lineage-specific genetic ablation

Lineage tracing and genetic ablation of ADAM12 - Nature

NettetGenetic technology using site-specific recombinases, such as the Cre-loxP system, has been widely employed for labeling specific cell populations and for studying their functions in vivo. To enhance the precision of cell lineage tracing and functional study, a similar site-specific recombinase system termed Dre-rox has been recently used in combination … NettetLineage-specific regulatory elements can be used to direct expression of a variety of genes to specific tissues in transgenic mice. If the hybrid constructs contain a gene …

Did you know?

NettetPrdm1 mutant mice are one of the rare mutant strains that do not develop whisker hair follicles while still displaying a pelage. Here, we show that Prdm1 is expressed at the earliest stage of whisker development in clusters of mesenchymal cells before placode formation. Its conditional knockout in the murine soma leads to the loss of expression of … Nettet14. apr. 2024 · Examination of lineage-specific markers identified clusters with gene signatures of osteoblast (cluster 4), osteocyte (cluster 5), adipocyte (cluster 7), and chondrocyte (cluster 8 and 9, Figure 1C). Chondrocyte clusters, while unexpected, likely represent growth plate chondrocytes released from bone fragments during enzymatic …

Nettet1. des. 2024 · Abstract. Through recurrent bouts synchronous with the hair cycle, quiescent melanocyte stem cells (McSCs) become activated to generate … Nettet1. jun. 2005 · Abstract. A new system for lineage ablation is based on transgenic expression of a diphtheria toxin receptor (DTR) in mouse cells and application of diphtheria toxin (DT). To streamline this approach, we generated Cre-inducible DTR transgenic mice (iDTR) in which Cre-mediated excision of a STOP cassette renders cells sensitive to DT.

Nettet1. nov. 2024 · Cell-specific expression of genes offers the possibility to use their promoters to drive expression of Cre-recombinase, thereby allowing for detailed … Nettet8. des. 2014 · Cell Ablations Can Confound Lineage Designations 662 Developmental Cell 31 , 654–667, December 8, 2014 ª 2014 Elsevier Inc. Using an extensive array of genetic approaches combined

Nettet26. apr. 2024 · Current research on cell fate mapping of endogenous CSCs largely relies on the conventional genetic lineage tracing technology that utilizes the promoters of specific markers expressed by CSCs. However, some proposed stem cell markers are nonspecific to putative CSCs, 12 , 19 – 21 as they are also expressed by cardiomyocytes.

Nettet19. apr. 2004 · To investigate the specificity and stability of lineage-specific gene expression, we included expression patterns of several continuous cell lines including Epstein–Barr virus-transformed lymphoblasts, T and B cell lymphomas, and control melanocyte cultures. We used Gene Ontology to select genes related to transcriptional … romantic gothic love quotesNettet2. apr. 2024 · 1 Introduction. Single-cell RNA-sequencing (scRNA-seq) technologies offer a chance to understand the regulatory mechanisms at single-cell resolution (Wen and Tang 2024).Subsequent to the technological breakthroughs in scRNA-seq, several analytical tools have been developed and applied towards the investigation of scRNA-seq data … romantic good night poetryNettetThe second was a genetically engineered ginip mouse that allows an inducible and tissue-specific ablation of GINIPexpressing sensory neurons in DRGs (Urien et al. 2024). ... romantic good night text for herNettet[Advances in lineage-specific genes] Yi Chuan. 2015 Jun;37(6):544-53. doi: 10.16288/j.yczz.14-392. [Article in Chinese] ... gene characterization, origin and … romantic gothic getawaysNettet3. apr. 2024 · Here we report the development of a brain-sparing DT, termed BRAINSPAReDT, for tissue-specific genetic ablation of cells outside the CNS. We … romantic goth dressesNettetthe genetic program is thought to account for the estab- lishment of cell lineages in multicellular organisms. Once different lineages are established, the cells of these line- … romantic goodnight paragraphs for herNettetAn epiblast-specific gene ablation revealed a novel specific threshold-dependent requirement for Sox2 during the epithelial-to-mesenchymal ... while Yap overexpression forces ESC to exit the cell cycle, driving lineage-specific differentiation. Thus, Yap1 levels orchestrate the balance between pluripotency and multipotency in ESCs ... romantic government