Dylight 550 vs pe
WebDyLight™ 405 - conjugated secondary antibodies are excited maximally at about 400 nm and fluoresce with a peak at about 421 nm. They are very bright and photostable, but their optimal use requires instruments … WebDyLight 550 562 576 4 PE* 496, 546 578 5 APC 650 661 4 Alexa Fluor 647 650 665 4 DyLight 650 654 673 4 PerCP 490 675 2 Alexa Fluor 700 Infrared 702 723 2 * PE is the …
Dylight 550 vs pe
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WebYes, the same experimental set-up can be used. 4. What is the excitation and emission of the DyLight® 550 fluorochrome? The excitation is 562nm and the emission is 576nm. As a comparison, the excitation of DyLight® … WebDyLight 550 NBP3-11552R: DyLight 594 NBP3-11552DL594: DyLight 650 NBP3-11552C: DyLight 680 NBP3-11552FR: DyLight 755 NBP3-11552IR: FITC NBP3-11552F: HRP NBP3-11552H: Janelia Fluor 549 NBP3-11552JF549: Janelia Fluor 646 NBP3-11552JF646: PE NBP3-11552PE: PerCP NBP3-11552PCP: Unconjugated NBP2-45313-0.1mg: View …
WebInvitrogen Dylight Antibody Labeling kits provide everything needed to label 1 mg of antibody or protein with amine-reactive Dylight dye and subsequent free dye removal. Choose from a variety of Dylight fluorophores across the spectrum to covalently label your antibody or protein. Labeled antibodies are typically ready to use in 90 minutes with ... WebH9 human embryonic stem (ES) cells were stained with either PE Mouse Anti-SSEA-4 (solid line) or PE mouse IgG3 (A112-3) isotype control (catalog number 559926, dashed line), incubated in the dark for 20 minutes at room temperature and analyzed by flow cytometry. Flow cytometry was performed on a BD FACSCalibur™ System.
WebBoth the absorption and emission properties of the DyLight® fluorochromes are compatible with the excitation and detection wavelengths of common fluorescence instrumentation. Our catalog includes secondary antibodies conjugated with DyLight ® 488, DyLight ® 550, DyLight ® 594 and DyLight® 650. Fig 1. WebDyLight 550 is brighter in most applications than TRITC, Cy3 & AlexaFluor 555. All Dylight 550 ... R-PE (Ex Max 496 nm / Em. Peak 578nm) R-phycoerythrin (R-PE) has a …
Web10 1040.06 0.139 mg of DyLight 550 NHS . Ester 150,000 MW 2 mg IgG × × = 13.9. μl of DyLight 550 NHS Ester 1 mg 100 μl 0.139 mg of DyLight 550 NHS Ester × = D. …
WebDyLight 550 NBP3-11552R: DyLight 594 NBP3-11552DL594: DyLight 650 NBP3-11552C: DyLight 680 NBP3-11552FR: DyLight 755 NBP3-11552IR: FITC NBP3-11552F: HRP NBP3-11552H: Janelia Fluor 549 NBP3-11552JF549: Janelia Fluor 646 NBP3-11552JF646: PE NBP3-11552PE: PerCP NBP3-11552PCP: Unconjugated NBP2-45313-0.1mg: View … how to shine up vinyl flooringWebThermo Scientific DyLight 550 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 550 used to fluorescently label sulfhydryl-containing … notre dame university newsWebDyLight 550 emits vibrant orange-to-red fluorescence for a wide array of fluorescence labeling-based applications. It has a strong absorption at 556nm, high fluorescence at 584nm (extinction coefficient 1.5 x105 cm-1M-1) and high quantum yield. ... with the exception of RPE and PE tandem dyes. For more details on the recommendations … notre dame university of ohioWeb10 1040.06 0.139 mg of DyLight 550 NHS . Ester 150,000 MW 2 mg IgG × × = 13.9. μl of DyLight 550 NHS Ester 1 mg 100 μl 0.139 mg of DyLight 550 NHS Ester × = D. Labeling Reaction . 1. To protect reagents from moisture, allow DyLight NHS Esters and DMF to equilibrate to room temperature before opening vials. 2. how to shine up tarnished silverwareWeb1/50 - 1/500. DyLight ® 550 replaces DyLight ® 549. The DyLight ® 550 excitation is 562nm and the emission is 576nm. DyLight ® 550 provides the same orange-to-red fluorescence and photostability as the DyLight ® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight ® 549 or DyLight ® 550 – this ... notre dame university san mateoWebAlexa Fluor® 555, ATTO 550, Cy®3, DyLight® 549, TRITC • Brighter than Cy®3 Validated in multicolor super-resolution imaging by STORM CF®568 562 583 532, 543, 546, 555, ... with R-PE, APC, Per-CP, R-PE-CF®750T, HRP, AP, and glucose oxidase CF® Dye SE and VivoBrite™ Protein Labeling Kits notre dame university shopWebBoster Bio Anti-Human CD2AP DyLight® 550 conjugated Antibody catalog # A01756-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human. … how to shine up vinyl plank flooring